热能与动力工程专业实验教学体系改革与实践

以谷氨酸棒杆菌(Corynebacterium glutamicum)CICC20887为生产菌株,采用单因素实验研究了发酵工艺条件对L-缬氨酸产量的影响.结果表明,该菌发酵生产L-缬氨酸的适宜初糖浓度、生物素添加量、VB1添加量、玉米浆添加量分别为90g/L、80μg/L、0.20mg/L、30g/L,发酵期间,24h前pH值应控制在6.5～6.7、后48h应控制在7.0～7.2,温度30～31℃,发酵周期应控制在66～72h.

Study on the fermentation process conditions of L-valine produced by Corynebacterium glutamicum

Using Corynebacterium glutamicum CICC20887 as producing strain,the effect of fermentation process condition on the yield of L-valine were studied with single factor design;The results showed that the optimum concentration of initial glucose,D-biotin,VB1 and corn syrup were 90g/L,80μg/L,0.20 mg /L,30g/L respectively;The optimal fermentation conditions of pH,temperature and fermentation period were 6.5~6.7(for the initial 24 hours),7.0~7.2(during 24 to 72 hours),30~31℃,66~72 h respectively.This study could offe a basis for industrial production of L-valine.