| 不同组织来源透明质酸的分离纯化及收率比较 |
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| 引用本文： | 张谦朋,赵子鹏,陈倩,赵隽咏. 不同组织来源透明质酸的分离纯化及收率比较[J]. 聊城大学学报(自然科学版), 2004, 17(1): 51-53 |
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| 作者姓名： | 张谦朋 赵子鹏 陈倩 赵隽咏 |
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| 作者单位： | 聊城大学,生命科学学院,山东,聊城,252059;冠县实验中学,山东,冠县,252500 |
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| 摘 要： | 以鸡冠和人脐带为原料，分别利用水浸提乙醇沉淀法以及酶解法提取透明质酸粗品．前者经DEAE-纤维素纯化得高纯度的透明质酸，后者经乙酸钠纯化得纯品．经测定，红外扫描图谱与文献报道一致．
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| 关 键 词： | 透明质酸 DEAE-纤维素 脐带 鸡冠 氯代十六烷基吡啶 |
| 文章编号： | 1672-6634(2004)01-0051-03 |
The Purification and Comparison of Production-rate of Hyaluronic Acid Isolated from Various Tissues |
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| ZHANG Qian-peng,ZHAO Zi-peng,CHEN Qian,ZHAO Juan-yong. The Purification and Comparison of Production-rate of Hyaluronic Acid Isolated from Various Tissues[J]. JOURNAL OF LIAOCHENG UNIVERSITY (NATURAL SCIENCE, 2004, 17(1): 51-53 |
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| Authors: | ZHANG Qian-peng ZHAO Zi-peng CHEN Qian ZHAO Juan-yong |
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| Affiliation: | ZHANG Qian-peng~ |
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| Abstract: | A pure high molecular weight hyaluronic acid was isolated from rooster comb and humen umbilcal cord seperately. The former's process invelved extraction with water, precipitation by ethanol. purification with DEAE-cellulose; the later's extraction with enzyme, precipitation also by thtanol. purification with Sodium acetate. By comparation the productivity from rooster comb is higher than from human umbilical cord. Their infrared spectrum analysis was in accord with that reported in the literature. |
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| Keywords: | hyaluronic acid DEAE-cellulose human umbilical cord rooster comb cetylpyridinium chloride |
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