Effect of salt concentration on binding of proteins to a non-ionic adsorbent |
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| Authors: | M Nemat-Gorgani K Karimian A R Massih |
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| Institution: | (1) Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 314-1700, Tehran, (Iran) |
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| Abstract: | Summary The effect of NaCl concentration on the adsorption of several proteins to palmityl-substituted Sepharose 4B has been investigated. It has been observed that the degree of adsorption first decreases and then increases with increasing salt concentrations, followed by total immobilization. The results are qualitatively explained by the simple theory of Debye-Hückel-Kirkwood, as applied to poly-electrolytes in the presence of salt.Acknowledgments. The authors wish to thank Ms H. Keshavarzi, Ms J. Nobakht and Mr F. Mohanazadeh for their technical assistance. |
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