| 热和碘乙酰胺灭活Saccharomyces carlsbergensis原生质体的融合 |
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| 引用本文： | 杜家福. 热和碘乙酰胺灭活Saccharomyces carlsbergensis原生质体的融合[J]. 河北大学学报(自然科学版), 1990, 0(3) |
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| 作者姓名： | 杜家福 |
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| 作者单位： | 河北大学生物系 |
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| 摘 要： | 本文选择了S. carlsbergensis Ⅰ和Ⅱ,分别用热和碘乙酰胺(CIA)双灭活原生质体,在聚乙二醇(PEGMW6000)诱导下进行融合,得到了双亲株融合子,并对形态观察、发酵性能、凝集性作了测定,以及对细胞可溶性蛋白质的聚丙烯酰胺凝胶电泳谱带进行了分析,认为获得的融合子具双亲特性。
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| 关 键 词： | 啤酒酵母 原生质体 灭活融合 |
Fusion of Heat and lodoacetamide Inactivated Protoplast of Saccharomyces Carlsbergensis |
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| Du Jiafu. Fusion of Heat and lodoacetamide Inactivated Protoplast of Saccharomyces Carlsbergensis[J]. Journal of Hebei University (Natural Science Edition), 1990, 0(3) |
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| Authors: | Du Jiafu |
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| Affiliation: | Department of Biology |
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| Abstract: | In this paper strains S. carlsbergensis Ⅰ and Ⅱ were selected, separately inactivated protoplast with heat and CIA, and then protoplast fusin with PEG induced, parental fusants were gained. Morphology was observed and fermentation and 'agglutination were measured. Soluble protein of yeast cell by discelectrophoresis iu polyacrylamide gel were compared. The experimental results showed that the obtained fusants possessing the parental feature. |
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| Keywords: | S. carlsbergensis Protoplasts Inactivated-fusion. |
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