| 血清中支原体污染的检测及其方法分析 |
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| 引用本文： | 李明生,冯玉萍,李倬,冯若飞,乔自林,周雪雁,马忠仁,陈红.血清中支原体污染的检测及其方法分析[J].西北民族学院学报,2006,27(4):37-40. |
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| 作者姓名： | 李明生 冯玉萍 李倬 冯若飞 乔自林 周雪雁 马忠仁 陈红 |
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| 作者单位： | 西北民族大学生命科学与工程学院 甘肃兰州730030 |
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| 摘 要： | 血清作为疫苗生产中的主要原辅材料,很容易被支原体污染.为了更好、更准确地检测血清中支原体的污染情况,本试验对血清中支原体污染的检测及方法进行了分析.分别用直接培养法、DNA荧光染色法和PCR法对17批新生牛血清样品进行了支原体检测.直接培养法检测结果有15批支原体阴性,2批阳性,阳性率为11.8%;DNA荧光染色法和PCR法检测结果均为14批支原体阴性,3批阳性,阳性率为17.6%.以上结果表明培养法与DNA荧光染色法或PCR法之间存在明显差异.对血清支原体进行检测时,应用直接培养法和DNA荧光染色法或PCR法同时联合应用,以提高对血清中支原体检测的准确性.
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| 关 键 词： | 血清 支原体 检测 |
| 文章编号： | 1009-2102(2006)04-0037-04 |
| 修稿时间： | 2006年10月20 |
Detection of Mycoplasma Contamination in Serum |
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| LI Ming-sheng,FENG Yu-ping,LI Zhuo,FENG Ruo-fei,QIAO Zi-lin,ZHOU Xue-yan,MA Zhong-ren,Chen Hong.Detection of Mycoplasma Contamination in Serum[J].Journal of Northwest Minorities University(Natural Science ),2006,27(4):37-40. |
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| Authors: | LI Ming-sheng FENG Yu-ping LI Zhuo FENG Ruo-fei QIAO Zi-lin ZHOU Xue-yan MA Zhong-ren Chen Hong |
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| Abstract: | To investigate the contamination situation of mycoplasmas in serum,the cultivatio,DNA fluorescent and polymerase chain reaction(PCR)were used respectively for detection of mycoplasmas in 17 batches calf serum. The results showed that 2 batches out of 17 batches calf serum was contained by mycoplasmas in detection of cultivation.The positive rate was 11.8%.However,there were both 3 positive in methods of DNA fluorescent and PCR in the same sample.Positive rate was 17.6%.It proved that there was significantly different between method of cultivation and DNA fluorescent or PCR.The cultivation combine with DNA fluorescent or PCR method for mycoplasmas detection can improve precision in batches calf serum. |
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| Keywords: | serum mycoplasma detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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