表面等离子体共振谱仪用于糖蛋白检测研究

介绍了一种表面等离子体共振(SPR)生化分析仪,采用角度扫描与强度调制相结合的方法来实现对糖蛋白的实时监测.在糖蛋白的检测实验中,对不同pH的再生液进行对比,pH=3的再生能力达到97%,选择该pH的溶液作为再生液;对同一浓度的糖蛋白进行5次测量,得出相对标准偏差(RSD)为3.83%;通过非糖蛋白与糖蛋白的对比实验,说明本实验方法可以对糖蛋白进行特异性检测;最后对浓度在0.01~1mg/mL的糖蛋白进行检测,并进行了线性拟合和多项式拟合,通过残差分析和相关系数的比较得出RNase B的浓度与归一化光强的关系更符合多项式拟合,因此以多项式拟合曲线作为其标准曲线.实验结果表明该SPR谱仪可以对糖蛋白进行特异性检测,该技术将会在医学方面得到广泛应用.

Research on detection of glycoprotein by surface plasmon resonance spectrometer

A surface plasmon resonance (SPR) biochemical analyzer is introduced. The method of combining angle scanning with intensity modulation is adopted to realize the real-time monitoring of glycoprotein. In the experiments of glycoprotein detection, the renewable solution of pH=3 is chosen because the regenerative capacity of the solution reaches 97% in the renewable solution of different pH comparison. Relative standard deviation (RSD) is 3.83% in the five measurements of the same concentration glycoprotein. The experimental method can detect the specificity of glycoprotein in the comparative experiments of non-glycoprotein and glycoprotein. Finally, the glycoprotein concentration between 0.01 mg/mL and 1 mg/mL is detected. The relationship between the concentration of RNase B and the normalized light intensity is more consistent with polynomial fitting by comparing the residual analysis and correlation coefficients of linear fitting and polynomial fitting, and polynomial fitting curve is used as standard curve. The experimental results show that the SPR spectrometer can detect the specificity of glycoprotein, while will be widely used in medical field.