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Detecting chimeric 5'l3'UTRs with cross-chromosomal splicing by bioinformatics |
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| Authors: | ZHANG Zhihua ZHANG Yong SHI Baochen DENG Wei ZHAO Yi CHEN Runsheng Institute of Biophysics Chinese Academy of Sciences Beijing China Institute of Computing Technology Chinese Academy of Sciences Bejing China Correspondence should be addressed to Chen Runsheng |
| Institution: | ZHANG Zhihua,,ZHANG Yong,,SHI Baochen,,DENG Wei,,ZHAO Yi,,CHEN Runsheng Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China Institute of Computing Technology,Chinese Academy of Sciences,Bejing 100080,China Correspondence should be addressed to Chen Runsheng |
| Abstract: | The 5'/3' UTRs of mRNA are crucial in transla- tional regulation, and several serious diseases are believed to be associated with abnormal splicing of these parts of the mRNA sequence. In this work a novel method which uses sequence alignment database searching for detecting chi- meric 5'3' UTRs with cross-chromosomal splicing is reported. Eight highly credible instances of cross-chromosomal splic- ing have been found using this method, representing addi- tional confirmation of the existence of cross-chromosomal splicing events provided by bioinformatics tools. Since no conserved motif has been found in any of the eight instances, and at the same time current prediction algorithms produce only trivial secondary structures at the "splicing sites", it is not possible to identify any specific signal leading to the splicing. |
| Keywords: | 5'/3' UTRs mRNA trans-splicing chromosomal translocations chimeric |
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