| 胶原酶灌注部分肝脏方法分离肝主质细胞 |
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| 引用本文： | 周家兴,夏民.胶原酶灌注部分肝脏方法分离肝主质细胞[J].河南师范大学学报(自然科学版),1990(1):96-100. |
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| 作者姓名： | 周家兴 夏民 |
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| 作者单位： | 河南师范大学生物系，河南师范大学生物系，河南师范大学生物系 |
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| 摘 要： | 本文报导一种分离哺乳类正常肝细胞的新方法,其要点是精确安排操作的程序,在灌注前结扎肝脏的左叶和正中叶,使灌注液只在余下的1/3肝脏内流动。本方法快速、稳定,能在2小时内从大鼠的1/3肝脏(2—3克肝组织)分离出1—1.5×10~7个和肝主质细胞,存活率在95%以上。酶液(0.05%胶原酶)的用量为20～25ml,在同类实验中是最少的。
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| 关 键 词： | 肝脏部分灌注 胶原酶 肝细胞分离 |
ISOLATION OF MAMMALIAN LIVER PARENCHYMAL CELLS BY COLLAGENASE PARTIAL LIVER PERFUSION METHOD |
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| Zhou Jiaxing,Xia Min,Jiang Ruiyun.ISOLATION OF MAMMALIAN LIVER PARENCHYMAL CELLS BY COLLAGENASE PARTIAL LIVER PERFUSION METHOD[J].Journal of Henan Normal University(Natural Science),1990(1):96-100. |
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| Authors: | Zhou Jiaxing Xia Min Jiang Ruiyun |
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| Institution: | Zhou Jiaxing;Xia Min;Jiang Ruiyun |
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| Abstract: | The essentials of the isolation method presented here are that all steps of manipulation were carefully
schedured,and two larger rat liver lobes were l:gated before perfusion.In gave 1-1.5×10~7 liver parenchymal cells from 2-3 grams of liver tissue(1/3 liver)in two hours.Both the purity and the vitality of harvested cells were no less than 95%.And the emzyme requirement redused to only 20-25 ml of.0.5% collagenase solution. |
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| Keywords: | partial livere prfusion collagenase isolation of liver cells |
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