| 冷冻电镜技术的应用与发展 |
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| 引用本文： | 张晓凯,张丛丛,刘忠民,刘力嘉,夏蕾.冷冻电镜技术的应用与发展[J].科学技术与工程,2019,19(24):9-17. |
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| 作者姓名： | 张晓凯 张丛丛 刘忠民 刘力嘉 夏蕾 |
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| 作者单位： | 山东师范大学物理与电子科学学院,济南,250014;实验室与设备管理处,济南,250014 |
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| 基金项目： | 国家自然科学基金项目(No.51173069);山东师范大学大型仪器设备开放(编号：KFJJ2017008), |
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| 摘 要： | 近年来,冷冻电镜技术获得了突飞猛进的发展,取得了许多具有开创性意义的成果,为科学研究起到了积极的推进作用。系统地综述了冷冻电镜技术与方法在科学研究中的应用,简要介绍了冷冻电镜技术的流程、发展、原理及解析方法,列举实例说明了冷冻电镜在结构生物学、生物医学、药物筛选方面的研究进展,分析了冷冻电镜技术与其他技术比较所具备的优越性以及存在的问题,并展望未来的发展。
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| 关 键 词： | 冷冻电镜 结构生物学 生物医学 药物筛选 |
| 收稿时间： | 2019/1/21 0:00:00 |
| 修稿时间： | 2019/6/26 0:00:00 |
Application and development of cryo electron microscopy technology |
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| Zhang xiaokai,Zhang congcong,Liu lijia and Xia lei.Application and development of cryo electron microscopy technology[J].Science Technology and Engineering,2019,19(24):9-17. |
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| Authors: | Zhang xiaokai Zhang congcong Liu lijia and Xia lei |
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| Institution: | School of physics and electronics, Management office of laboratory and equipment,Shandong Normal University,Jinan,School of physics and electronics, Management office of laboratory and equipment,Shandong Normal University,Jinan,,School of physics and electronics, Management office of laboratory and equipment,Shandong Normal University,Jinan,School of physics and electronics, Management office of laboratory and equipment,Shandong Normal University,Jinan |
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| Abstract: | In recent years, the rapid development of cryo electron microscopy has been made by leaps and bounds, and many pioneering achievements have been achieved, which have played an active role in scientific research. The application of cryo electron microscopy techniques and methods in scientific research is summarized, and the process, development, principle and analytical methods of cryo electron microscopy are briefly introduced. The progress of cryo electron microscopy in structural biology, biomedicine and drug screening is illustrated by examples. Compared with other technologies, the advantages and existing problems of cryo electron microscopy and its future development are also pointed out. |
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| Keywords: | Cryo-electron microscopy structural biology biomedicine drug screening |
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