| 植物血球凝集素-Sepharose 4B亲和层析纯化人绒毛膜促性腺激素 |
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| 引用本文： | 吴丹奇,方桦.植物血球凝集素-Sepharose 4B亲和层析纯化人绒毛膜促性腺激素[J].汕头大学学报(自然科学版),1998,13(1):32-35. |
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| 作者姓名： | 吴丹奇 方桦 |
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| 作者单位： | 汕头大学生物学系!汕头，515063，汕头大学生物学系!汕头，515063，汕头大学生物学系!汕头，515063 |
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| 摘 要： | 本文报道一种新的制备和纯化HCG的方法．人工流产物经45％乙醇抽提、调pH5．2～5．4后40～60％乙醇分级、植物血球凝集素-Sepharose4B柱层析纯化后，低温酒精沉淀干燥，可得到比效价3777.8IU／mg的hCG精品．
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| 关 键 词： | 亲和层析 植物血球凝集素 人绒毛膜促性腺激素 |
Purification of human chorionic gonadotropin with phytohemagglutinin affinity chromatography |
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| Wu Danqi,Fang Hua,He Zhirong.Purification of human chorionic gonadotropin with phytohemagglutinin affinity chromatography[J].Journal of Shantou University(Natural Science Edition),1998,13(1):32-35. |
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| Authors: | Wu Danqi Fang Hua He Zhirong |
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| Abstract: | In this paper is reported a new method for preparation and purification of human chorionic gonadotropin (HCG) from artificial abortion. Crude HCG was purified by 45 % alcohol extraction, then by 40~60 % alcohol fractionation at pH5. 2~ 5. 4, and then by the phytohemagglutinin (PHA ) sepharose 4B affinity chromatography and the precipitation of affinity eluant by alcohol at low temperature to obtain refined HCG whose immunizing potency is 3777. 8 IU/mg. |
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| Keywords: | affinity chromatography phytohemagglutinin (PHA ) human chorionic gonadotropin (HCG) |
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