A new,rapid and precise method for measuring the intra-cellular water content by gas chromatography and3H-sucrose |
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| Authors: | K Kageyama T Yamashita T Kobayashi E Kimura |
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| Institution: | (1) Department of Physiology and Pediatrics, Osaka City University Medical School, Abeno-ku, 545 Osaka, (Japan) |
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| Abstract: | Summary A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26±0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood. |
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