| 纳豆激酶纯化及性质研究 |
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| 引用本文： | 陆婕,付正纲,陈正望.纳豆激酶纯化及性质研究[J].华中科技大学学报(自然科学版),2005,33(11):115-117. |
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| 作者姓名： | 陆婕 付正纲 陈正望 |
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| 作者单位： | 华中科技大学,生命科学与技术学院,湖北,武汉,430074;华中科技大学,生命科学与技术学院,湖北,武汉,430074;华中科技大学,生命科学与技术学院,湖北,武汉,430074 |
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| 基金项目： | 国家自然科学基金资助项目（30370647）. |
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| 摘 要： | 以蒸煮后的大豆为原料通过纳豆菌发酵,生产纳豆激酶.改进纯化工艺以酸法提取和凝胶过滤纯化出比活性为160.8IU/mg的酶制品.该酶制品不仅对纤维蛋白有很好的溶纤作用,而且对大鼠血纤维（拟血栓）也有很好的溶纤活性,实验还证明该酶制品对大鼠新鲜血液有很好的抗凝性能,尤其是该酶制品在pH值为2.45和经胃蛋白酶作用后仍保持较高的活力.同时,该方法制备工艺简便,有0.6%的高回收率,故本方法提取的纳豆激酶制品有望开发成为新型口服溶栓、防栓制剂,以供医疗和保健应用.
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| 关 键 词： | 纳豆激酶 溶栓 抗凝 |
| 文章编号： | 1671-4512（2005）11-0115-03 |
| 收稿时间： | 2005-01-25 |
| 修稿时间： | 2005年1月25日 |
Study of the rough purification of nattokinase and its character |
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| Lu Jie,Fu Zhenggang,Chen Zhengwang.Study of the rough purification of nattokinase and its character[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2005,33(11):115-117. |
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| Authors: | Lu Jie Fu Zhenggang Chen Zhengwang |
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| Abstract: | The preparation of nattokinase by fermenting soybeans was studied.Using the improved purified procedures with acid extraction and gel filtration(Sephadex G-25 fine),the specific activity of the product(160.80?IU/mg) and the recovering rate from the initial material soybeans(0.6?%) were enhanced compared to the reported products.The preparation in this study showed the both of biofunctions were not only in fibrinolysin,also in anticoagulation.Especially the nattokinase could still display its bioactivity at pH 2.45 and after digestion with pepsin,illustrating that this preparation might be used as oral administration.The mechanisms of the actions are deserved to be further investigated. |
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| Keywords: | nattokinase fibrinolysis anticoagulant |
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