纤维素酶蛋白质工程的新进展

原始纤维素酶通常存在催化活性低下、末端产物抑制、热稳定性不高、持续催化能力弱等问题，因此，改善它的酶学性质是很有意义的工作。目前，采用体外进化技术是改进纤维素酶酶学性质的一种有效途径。从纤维酶的催化域、结合域和连接区等不同区域出发，综述了纤维素酶蛋白质工程的新进展。提出了系统组装3个已改造功能结构域的思想，这有助于提高纤维素酶催化活性、持续催化能力、耐高浓度产物、热稳定性等特性，将促进纤维素酶在能源、工业、农业、食品等领域的高效应用。

The Recent Advances in Protein Engineering of Cellulases

Parent cellulases exist in some defects of a low catalytic activity,inhibition of end products,weak thermo-stability,and poor processivity,and therefore improving enzymatic properties of cellulases is a very meaningful job. To date,on the basis of the technology of evolution in vitro,it is an effective way to develop enzymatic properties of cellulases. Recent advances in protein engineering about three different regions of cellulases which consist of catalyt-ic domains,binding domains and linker regions are reviewed in the paper. The idea about systematically assembling functional structure domains which have been engineered was put forward to in the paper,which will help to improve the enzymatic properties such as catalytic activity,the ability of processive hydrolysis,resistantance to high concen-tration of product,and thermalstability,and to promote high-efficent application in energy,industry,agriculture, food,and so on.