| 蜡质芽孢杆菌酰基高丝氨酸内酯酶基因的克隆及序列分析 |
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| 引用本文： | 黄天培,潘洁茹,姚帆,黄张敏. 蜡质芽孢杆菌酰基高丝氨酸内酯酶基因的克隆及序列分析[J]. 武夷科学, 2007, 23(1): 29-34 |
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| 作者姓名： | 黄天培 潘洁茹 姚帆 黄张敏 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002 |
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| 基金项目： | 福建省青年科技人才创新基金;福建省自然科学基金 |
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| 摘 要： | 利用pMD18-T克隆载体从蜡质芽孢杆菌菌株T—HW3中克隆了酰基高丝氨酸内酯酶基因（AHL—lactonase，aiiA）。测序结果表明，该基因（GenBank登录号为DQ000643）由753个碱基组成，编码含有250个氨基酸残基的蛋白质。该蛋白质的推测的分子量为28kDa，等电点为4．235左右。核苷酸序列的BLAST分析结果表明，与之同源性较高的基因均为蜡质芽孢杆菌组aiiA基因（86％-99％）。
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| 关 键 词： | 蜡质芽孢杆菌 酰基高丝氨酸内酯酶基因 基因克隆 序列分析 |
| 文章编号： | 1001-4276-（2007）01-0029-06 |
| 收稿时间： | 2007-09-13 |
| 修稿时间： | 2007-09-13 |
CLONING AND SEQUENCE ANALISIS OF AIIA GENE FROM BACILLUS CEREUS |
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| HUANG Tian-pei,PAN Jie-ru,YAO Fan,HUANG Zhang-min. CLONING AND SEQUENCE ANALISIS OF AIIA GENE FROM BACILLUS CEREUS[J]. Wuyi Science Journal, 2007, 23(1): 29-34 |
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| Authors: | HUANG Tian-pei PAN Jie-ru YAO Fan HUANG Zhang-min |
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| Affiliation: | Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education, Fuzhou, Fujian 350002, China |
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| Abstract: | A novel aiiA was cloned from Bacillus cereus T - HW3 by PCR with pMD18 - T vector and was sequenced. The nucleotide sequence had been registered in GenBank with accession No. 3. It consists of 753 bases which encoded a polypeptide of 250 amino acids residues with a calculated molecular weight of 28 kDa and an isoelectric point value of 4. 235. BLAST analysis of its nucleotide sequence showed 86% -99% identity with those of the aliA genes from B. cereus group. |
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| Keywords: | Bacillus cereus aliA cloning sequence analysis |
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