制备色谱法在纯化甜茶苷工艺中的应用

研究了从甜茶叶中纯化甜茶苷的制备工艺。首先用沸水从甜茶叶中提取甜茶苷，提取3次，每次1 h。再利用HP-20大孔吸附树脂对甜茶苷粗提物进行预纯化，以90%乙醇为洗脱液，洗脱6倍柱体积；浓缩去除乙醇后，用D-941阴离子交换树脂脱色，水洗并干燥得到甜茶苷粗产品，纯度为68.6%。最后甜茶苷粗产品经制备色谱纯化后，得到纯度为97.9%的甜茶苷，色谱条件为流动相V_甲醇 ∶V_水=70∶30，流速10mL/min，上样量200 mg。

The use of preparative HPLC in the purification of rubusoside from Rubus suavissimus S.Lee leaves

The preparative separation of rubusoside from the leaves of Rubus suavissimus S. Lee has been investigated. First, the leaves were extracted three times using boiling water, with an extraction time of 1 h. Second, HP-20 macroporous resins were used in the pre-purification procedure, with 6BV of 90% ethanol in water used as the eluent. Third, the solution was evaporated to remove ethanol and loaded onto pretreated D-941 anion-exchange resin for decoloration. Finally, preparative HPLC was used for further purification. Rubusoside with a purity of 97.9% was obtained from the 68.6% purity rubusoside extract after the preparative HPLC using 70% methanol in water as the mobile phase, a flow rate of 10 mL/min and a sample loading of 200 mg.