DNA条形码技术在白洋淀流域浮游动物调查中的应用

DNA 条形码在物种鉴定中得到了有效的应用,有助于分类学和生物多样性研究. 本研究基于线粒体COI基因序列,使用邻接法(neighbor joining method,NJ)和最大似然法(maximum Likelihood,ML)构建系统发育树,对2018年5月在白洋淀流域采集的浮游动物样品进行初步研究. 结果表明,在对桡足类的系统发育树分析时,NJ树和ML树的结果保持一致,而且和形态学鉴定结果保持一致. 在对枝角类的系统发育树的分析中,NJ树和ML树的结果有所差异,和形态学鉴定结果也有所不同,分析原因,可能是由于拼接序列时剪短了部分序列,使序列间差异性变小引起误差. 总体上看,基于COI基因的DNA条形码可以有效地应用到白洋淀流域的浮游动物鉴定中,并可以逐步完善,最终建立一个完整的DNA条形码数据库.

Application of DNA barcode on survey of zooplankton in Baiyangdian Watershed

DNA barcode has been effectively used in identification of species, for taxonomy also biodiversity research. This study constructs phylogenetic trees,which based on mitochondrial COI gene sequences, using adjacency method(neighbor joining method, NJ)and maximum likelihood method(maximum likelihood, ML). It was a preliminary study on the acquisition of zooplankton samples in Baiyangdian Watershed in May 2018. The results showed that the phylogenetic tree analysis of copepods, NJ tree and the tree of ML is consistent with the results of morphological identification results. On the tree branch angle of system development analysis, the result of the NJ tree and ML tree was also different with the result of morphological identification. The reason may be due to the stitching part cut short sequences, the sequence error caused by smaller differences. Overall, based on the COI gene, DNA barcode can be effectively applied to zooplankton in Baiyangdian Watershed and it can gradually improve the identification and finally to create a complete DNA barcode database.