| 温和化学渗透剂对解脂假丝酵母神经酰胺含量的影响 |
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| 引用本文： | 赵静. 温和化学渗透剂对解脂假丝酵母神经酰胺含量的影响[J]. 青海大学学报, 2006, 24(1): 44-46 |
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| 作者姓名： | 赵静 |
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| 作者单位： | 青海大学农牧学院,青海,西宁,810003 |
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| 摘 要： | 为了分析温和化学渗透剂木瓜蛋白酶、甘氨酸对解脂假丝酵母33M Candida lipolytica中神经酰胺含量的影响，分别采用氯仿甲醇抽提法和乙醇抽提法提取神经酰胺，用固相萃取法（SPE）分离神经酰胺与其它膜中脂类，并用薄层色谱（TLC）方法和高效液相色谱-蒸发光散射器（HPLC-ELSD）对其进行定性和定量分析，结果表明：加温和化学渗透剂木瓜蛋白酶、甘氨酸能增加解脂假丝酵母中的神经酰胺含量，并以木瓜蛋白酶效果最好。
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| 关 键 词： | 解脂假丝酵母 神经酰胺 固相萃取法 薄层色谱法 高效液相色谱-蒸发光散射器 |
| 文章编号： | 1006-8996（2006）01-0044-03 |
| 收稿时间： | 2005-09-13 |
| 修稿时间： | 2005-09-13 |
Effect on ceramids contents of Candida lipolytica by mildchemical permeability agents |
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| ZHAO Jing. Effect on ceramids contents of Candida lipolytica by mildchemical permeability agents[J]. Journal of Qinghai University(Natural Science), 2006, 24(1): 44-46 |
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| Authors: | ZHAO Jing |
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| Affiliation: | Agriculture and Animal Husbandry College, Qinghai University, Xining 810003, China |
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| Abstract: | Effect on ceramids of Candida lipolytica of glucine and papain were analysed. Ceramids was extracted by chloroform and alcohol method respectively. In order to acquire better chromatography result and protect the elution column, SPE was applied to isolate ceramids from other membrane lipids and TLC and HPLC- ELSD were applied for quantitative and qualitative analysis respectively. The result showed that glucine and papain increased ceramids contents. |
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| Keywords: | Candida lipolytica ceramids SPE TLC HPLC - ELSD |
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