| 橡椀单宁降解酶特性的研究 |
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| 引用本文： | 黄文,石碧.橡椀单宁降解酶特性的研究[J].北京大学学报(自然科学版),2003,39(4):579-582. |
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| 作者姓名： | 黄文 石碧 |
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| 作者单位： | 北京大学环境工程系，水沙科学教育部重点实验室，北京，100871；四川大学皮革化学与工程教育部重点实验室，成都，610065 |
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| 基金项目： | 国家自然科学基金;29872025; |
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| 摘 要： | 借助于没食子酸丙酯为模型化合物作为底物，对一株能抵抗橡椀单宁(鞣花类单宁)生物毒性并能有效降解这种单宁的菌株的降解特性进行了研究。结果表明这株菌不仅具有单宁酶的活性，而且具有多酚氧化酶（PPO）的活性。通过对不同底物对内胞霉PPO酶促反应速度的影响研究证实橡椀单宁的降解不仅与单宁酶有关还与多酚氧化酶有关。
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| 关 键 词： | 没食子酸丙酯 橡椀单宁 鞣花单宁 单宁酶 多酚氧化酶 |
| 收稿时间： | 2003-02-10 |
Characters of Valonia Tannin Degrading-Enzymes from Endomyces |
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| HUANG Wen,SHI Bi.Characters of Valonia Tannin Degrading-Enzymes from Endomyces[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2003,39(4):579-582. |
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| Authors: | HUANG Wen SHI Bi |
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| Institution: | Dept. of Environmental Engineering, College of Environmental Sciences, Peking University; The Key Lab of Water and Sediment Sciences, Ministry of Education, Beijing, 100871; The Key Lab of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065 |
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| Abstract: | Characters of degrading enzymes from Endomyces, which can resist toxici ty of valonia tannin (ellagitannin) and degrade tannin effectively, using propyl gallate instead of valonia tannin as substrate were investigated. It was found that the strain have not only activity of tannase, but also activity of polyphen ol oxidase(PPO). By using model compound, catechol and pyrogallol, it was verified that at least both tannase and PPO were involved during degradat ion of tannins by the strain. |
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| Keywords: | propyl gallate valonia tannin ellagictannin tannas e polyphenol oxidase |
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