| 秀丽线虫sod-3基因RNA干扰及早衰模型的建立 |
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| 引用本文： | 关树文,黄磊,罗晶,韩秋菊,王丽萍,李惟. 秀丽线虫sod-3基因RNA干扰及早衰模型的建立[J]. 吉林大学学报(理学版), 2007, 45(4): 676-680 |
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| 作者姓名： | 关树文 黄磊 罗晶 韩秋菊 王丽萍 李惟 |
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| 作者单位： | 吉林大学,生命科学学院,长春,130021;东北师范大学,遗传与细胞研究所,长春,130021 |
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| 基金项目： | 吉林省长春市科技攻关计划 , 长春市与吉林大学生命科学学院合作计划项目 |
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| 摘 要： | 利用RNAi技术， 通过干扰秀丽线虫中的sod-3基因表达， 建立了因超氧化物歧化酶3（SOD3）表达缺失引起快速衰老的线虫模型，为sod-3 基因功能的研究以及相关疾病的基因治疗等提供动物模型.
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| 关 键 词： | RNAi 秀丽线虫 超氧化物歧化酶3 |
| 文章编号： | 1671-5489（2007）04-0676-05 |
| 收稿时间： | 2006-09-19 |
| 修稿时间： | 2006-09-19 |
Model of Rapid Aging Based on RNAi-Mediated Gene Silencing of sod-3 Expression in Caenorhabditis Elegans |
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| GUAN Shu-wen,HUANG Lei,LUO Jing,HAN Qiu-ju,WANG Li-ping,LI Wei. Model of Rapid Aging Based on RNAi-Mediated Gene Silencing of sod-3 Expression in Caenorhabditis Elegans[J]. Journal of Jilin University: Sci Ed, 2007, 45(4): 676-680 |
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| Authors: | GUAN Shu-wen HUANG Lei LUO Jing HAN Qiu-ju WANG Li-ping LI Wei |
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| Affiliation: | 1. Collge of Life Science, Jilin University, Changchun 130021, China; 2. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, China |
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| Abstract: | According to the oxidative damage theory of aging, free radicals, particularly reactive oxygen species (ROS), are responsible for the senescence and many human diseases. ROS are generated during normal metabolic process in all the oxygen utilizing organisms. They are detoxified by superoxide dismutase. The manganese containing superoxide dimutase 3 (SOD3) has been shown to play a role in the aging process in Caenorhabditis elegans. In this research, we show that sod-3 RNA interference (RNAi) inducesinsenecencein Caenorhabditis elegansas expected. This method can be used to understand the process of aging and the mechanism of antioxidance. |
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| Keywords: | RNAi Caenorhabditis elegans superoxide dismutase 3 |
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