一株多氯联苯降解菌的分离鉴定及基因分型

从深海底泥中分离筛选出一株以多氯连苯（PCBs）为惟一碳源和能源生长的菌株， 命名为pdi. 该菌在含有PCBs 7.5 mg/L的150 mL液体培养基中培养7d， 用GC MS检测其对PCBs的降解率达95.38%. 以细菌16SrDNA通用引物对pdi基因组进行PCR扩增， 得到~1　500　bp的片段. 经纯化， 测序后在Genbank上进行同源性比较分析及系统发育树构建， 该菌与Pseudomonsa spTS1138同源性达100%， 初步鉴定该菌为假单胞菌属. 对pdi基因组进一步运用RAPD分子标记技术进行随机引物扩增基因分型， 获得了稳定的DNA 指纹图谱.

Identification and Phylogenetic Analyses of a PCBs Degrading Strain

A strain pdi which can use biphenyl as the sole carbon source was isolated from seabed mud and cultured for 7 dwith 150 mL of fluid medium which contained 7.5 mg/L polychlorinated biphenyls (PCBs).　GC MS measurement showed PCBs degradation efficiency was 95.38%. The genome was amplified with bacterium 16SrDNA primer. The amplified product (about 1 500 bp) was purified and sequenced for phylogenetic analyses with Genbank. The bacterium pdi was identified as Pseudomonsa spTS1138. RAPD analyses of pdi produced stable DNA fingerprint. These studies have provided parameter for the further dynamic resolution of bacterium clusters for the rehabilitation of PCBs contaminated underground water environment with pdi PRB method.