| 洁霉素产生菌原生质体的形成、再生和融合 |
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| 引用本文： | 林莺.洁霉素产生菌原生质体的形成、再生和融合[J].华侨大学学报(自然科学版),1989,0(3):331-336. |
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| 作者姓名： | 林莺 |
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| 作者单位： | 华侨大学化工与生化工程系 |
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| 摘 要： | 洁霉素产生菌不论生长在含有甘氨酸的S培养基或不含甘氨酸的S培养基的菌丝中,其细胞壁均可被溶菌酶溶解而释放出原生质体。甘氨酸浓度为1.0%破壁效果最好,溶菌酶的作用浓度以2.5mg/ml为最佳。原生质体在Hb培养基上能够很好地再生。用P培养基配制的40%(w/v)PEG1000溶液,能有效地诱导洁霉素产生菌原生质体融合。融合重组频率达6.7%。
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| 关 键 词： | 洁酶素 原生质体 溶菌酶 |
The Formation, Regeneration and Recombination of Protoplasts from Lincomycinum Productive Strain |
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| Lin Ying.The Formation, Regeneration and Recombination of Protoplasts from Lincomycinum Productive Strain[J].Journal of Huaqiao University(Natural Science),1989,0(3):331-336. |
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| Authors: | Lin Ying |
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| Abstract: | This paper studies the formation, regeneration and recombination of proto- plasts from the mycelia of lincomycinum productive strain which grown on S medium containing glycine or not. The cell wall of mycelia can be solved by ly- sozyme and thus protoplasts liberated. 1.0% glycine in S medium and 2.5mg/ml lysozyme are the optimum concentration for its liberation. The protoplasts rege- nerate very well on Hb medium. The protoplasts recombinate effectively by fu- sion in 40% (w/v) PEG 1,000 solution prepared with P medium, with a recom- bination ratio of 6.7%. |
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| Keywords: | lincomycinum protoplast lysozyme |
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