| PCR-DGGE法研究Sludge bio-membrane(SB)系统中反硝化聚磷菌的变化 |
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| 引用本文： | 刘晖,孙彦富,周康群,顾雪婷,刘洁萍,陈捷美.PCR-DGGE法研究Sludge bio-membrane(SB)系统中反硝化聚磷菌的变化[J].中南大学学报(自然科学版),2011,42(4). |
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| 作者姓名： | 刘晖 孙彦富 周康群 顾雪婷 刘洁萍 陈捷美 |
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| 作者单位： | 仲恺农业工程学院环境科学与工程系,广东广州,510225 |
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| 基金项目： | 国家自然科学基金，广东自然科学科学基金，广东省教育厅项目，广州市海珠区科技计划项目 |
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| 摘 要： | 采用SB同步脱氮除磷系统富集反硝化聚磷菌,利用平板分离法和PCR-DGGE技术,进行微生物种群的跟踪.研究结果表明:通过平板法分离到的微生物主要为棒状杆菌属、不动杆菌属、假单胞菌属、肠杆菌科、莫拉氏菌属、葡萄球菌属、副球菌属共7种,富集后细菌主要为不动杆菌属、假单胞菌属、肠杆菌科、副球菌属4种,富集后系统内细菌种类减少,与采用Sequencing Batch Reactor(SBR)池富集的反硝化聚磷菌不同.采用PCR-DGGE法发现富集前以黄杆菌属、产碱杆菌属、副球菌属、紫色杆菌属、赤细菌属为主,富集后以产碱杆菌属、副球菌属、紫色杆菌属为主,副球菌属是唯一通过2种办法获得确认的反硝化聚磷菌株.采用PCR-DGGE和16S rDNA克隆文库方法研究的同步反硝化聚磷菌都以变形门占优势.
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| 关 键 词： | 反硝化聚磷菌 细菌 PCR-DGGE法 SB系统 |
Change of denitrifying phosphate bacteria(DPB) in sludge bio-membrane system using PCR-DGGE method |
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| LIU Hui,SUN Yan-fu,ZHOU Kang-qun,GU Xue-ting,LIU Jie-ping,CHEN Jie-mei.Change of denitrifying phosphate bacteria(DPB) in sludge bio-membrane system using PCR-DGGE method[J].Journal of Central South University:Science and Technology,2011,42(4). |
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| Authors: | LIU Hui SUN Yan-fu ZHOU Kang-qun GU Xue-ting LIU Jie-ping CHEN Jie-mei |
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| Institution: | LIU Hui,SUN Yan-fu,ZHOU Kang-qun,GU Xue-ting,LIU Jie-ping,CHEN Jie-mei (Department of Environmental Science and Engineering,Zhongkai University of Agricultural and Engineering,Guangzhou 510225,China) |
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| Abstract: | Sludge bio-membrane nitrogen and phosphorus removal system was used to enrich denitrifying phosphorus bacteria(DPB).Plate separation and PCR-DGGE technology were applied to monitor the diversity of bacteria.The results show that before the enrichment,isolated micro-organisms are mainly seven kinds of bacteria including Corynebacterium sp.,Acinetobacter sp.,Pseudomonas sp.,Enterobacteriaceae sp.,Moraxella sp.,Staphylococcus sp.and Paracoccus sp.,seven kinds of bacteria are isolated by the plate separation.Af... |
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| Keywords: | denitrifying phosphate bacteria(DPB) bacteria PCR-DGGE sludge bio-membrane system |
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