硫酸二甲酯对患丛枝病毛泡桐蛋白组的影响

【目的】找出与泡桐丛枝病发生相关的蛋白,为研究泡桐丛枝病发病机制提供依据。【方法】利用iTRAQ技术对使用硫酸二甲酯(DMS)处理后恢复健康的毛泡桐患丛枝病苗、未经处理的毛泡桐病苗和健康苗进行蛋白质组学分析。【结果】共鉴定得到2 628个蛋白质,其中177个蛋白表达水平具有显著差异; 通过比对分析找到37个与泡桐丛枝病发生密切相关的差异表达蛋白。代谢途径分析可知,37个差异表达蛋白主要参与光合作用、植物-病原物相互作用、氧化磷酸化等途径。【结论】毛泡桐感染泡桐丛枝病过程中,与光合作用相关的蛋白受到影响,钙调蛋白(CaM)在植原体与毛泡桐的相互作用过程中发挥作用。

Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma

【Objective】Determine the proteins related to Paulownia broom disease(PaWB)and provide a basis for studying its pathogenesis. 【Method】We used isobaric tags for relative and absolute quantitation(iTRAQ)to analyze proteomes for PaWB-infected Paulownia tomentosa seedlings treated and untreated with dimethyl sulphonate(DMS), and healthy seedlings as the control.【Result】A total of 2 628 proteins was identified, among which 177 proteins exhibited differential expression. By comparison, 37 differentially expressed proteins were found to relate to Paulownia witches' broom disease. Pathway analysis showed that these 37 proteins were mainly involved in photosynthesis, plant-pathogen interactions, and oxidative phosphorylation pathways. 【Conclusion】During the process of PaWB occurrence, the proteins related to photosynthesis were affected. CaM was involved in the interaction between phytoplasma and Paulownia. These results have important significance for the further study of the mechanism of Paulownia witches' broom disease at the protein level.