| 甘蔗UGPase cDNA的克隆及序列分析 |
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| 引用本文： | 连玲,叶冰莹,何炜,张积森,何文锦,陈由强,陈如凯.甘蔗UGPase cDNA的克隆及序列分析[J].福建师范大学学报(自然科学版),2009,25(3). |
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| 作者姓名： | 连玲 叶冰莹 何炜 张积森 何文锦 陈由强 陈如凯 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108;发育与神经生物学福建省高等学校重点实验室,福建,福州,350108
2. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108 |
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| 基金项目： | 引进国际先进农业科学技术计划(948计划)，农业公益性行业科研专项基金 |
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| 摘 要： | 以甘蔗(FN95-1702)为材料,通过RT-PCR,首次克隆得到了甘蔗UGPase cDNA片段.该片段长1 495 bp,其中包含完整的ORF为1 431 bp,共编码476个氨基酸,并含有5个重要的Lys残基位点,分别为Lys257、Lys321、 Lys367、 Lys408、 Lys409,它们对维持UGPase活性及与底物结合方面发挥着重要作用.
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| 关 键 词： | 基因克隆 序列分析 |
Cloning and Sequence Analysis of UDP-glucose Pyrophosphorylase cDNA from Saccharum officinarum |
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| LIAN Ling,YE Bing-ying,HE Wei,ZHANG Ji-sen,HE Wen-jin,CHEN You-qiang,CHEN Ru-kai.Cloning and Sequence Analysis of UDP-glucose Pyrophosphorylase cDNA from Saccharum officinarum[J].Journal of Fujian Teachers University(Natural Science),2009,25(3). |
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| Authors: | LIAN Ling YE Bing-ying HE Wei ZHANG Ji-sen HE Wen-jin CHEN You-qiang CHEN Ru-kai |
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| Institution: | 1.College of Life Sciences;Fujian Normal University;Fuzhou 350108;China;2.Key Laboratory of Eco-physiology and Genetics Improvement for Sugarcane;Ministry ofAgriculture;3.State Key Laboratory of Developmental Biology and Neruobiology;China |
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| Abstract: | A cDNA encoding UGPase was isolated from the leaf of Saccharum officinarum by RT-PCR,the sequence consists of 1 495 bp,has an open reading frame of 1 431 bp and encodes a protein of 476 amino acids.And the amino acid sequence contains five important Lys residues(Lys257,Lys321,Lys367,Lys408,Lys409) which are critical for catalysis and substrate binding to the enzyme. |
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| Keywords: | UGPase |
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