| 微过氧化物酶-11在多壁碳纳米管修饰玻碳电极上的电化学 |
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| 引用本文： | 王琨琦,马中苏. 微过氧化物酶-11在多壁碳纳米管修饰玻碳电极上的电化学[J]. 长春工程学院学报(自然科学版), 2008, 9(4): 80-83 |
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| 作者姓名： | 王琨琦 马中苏 |
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| 作者单位： | 长春工程学院,理学院,长春,130012;吉林大学生物与农业工程学院,长春,130025;吉林大学生物与农业工程学院,长春,130025 |
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| 基金项目： | 国家自然科学基金资助 |
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| 摘 要： | 利用物理吸附的方法将微过氧化物酶-11（MP-11）固定在多壁碳纳米管（MCNT）修饰的玻碳电极表面。研究发现：在pH=7.12磷酸盐缓冲溶液中，修饰电极上的MP-11发生了两电子一质子准可逆的氧化还原反应，式量电位E0’为-298mV（vs Ag/AgCl），峰电位差ΔEP为39mV。在该修饰电极上MP-11对氧气（O2）和过氧化氢（H2O2）都能进行催化还原，催化还原过程都是扩散控制的过程。而且在信噪比为3时，MP-11对H2O2的最低检出限是0.35mmol/L，表明MP-11在修饰电极上保持了自身的生物活性，该修饰电极有望在生物燃料电池和生物传感器中得到应用。
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| 关 键 词： | 微过氧化酶-11 多壁碳纳米管 电催化 |
Electrochemistry of microperoxidase-11 on a glass carbon electrode modified with multi wall carbon nanotubes |
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| WANG Kun-qi,et al.. Electrochemistry of microperoxidase-11 on a glass carbon electrode modified with multi wall carbon nanotubes[J]. Journal of Changchun Institute of Technology(Natural Science Edition), 2008, 9(4): 80-83 |
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| Authors: | WANG Kun-qi et al. |
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| Affiliation: | WANG Kun- qi, et al. (Faculty of Science, Changchun Institute of Technology, Changchun 130012, China) |
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| Abstract: | Microperoxidase - 11 （ MP - 11 ） is immobilized on a glassy carbon electrode （GCE） modified with multiwall carbon nanotubes by physical adsorption method. It was found that MP - 11 on the modified electrode takes place a quasi - reversible two electron redox reaction with a formal potential （E0＇） of - 298mV and AEP is 39mV in phosphate buffer solution （PBS） （pHT. 12）. The MP- 11on the modified electrode can catalyze the reduction O2 and H2O2. The catalytic process of O2 and H2O2 are diffusion - controlled process. The detection limit of H2O2 is 0.35mmol/L at the signal to noise ratio of three. It is obvious that bioactivity of MP - lion the modified electrode with MWCNTs is kept. The modified electrode can be used in the biofuell cell and biosensor in the future. |
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| Keywords: | microperoxidase-11 multi wall carbon nanotubes electrocatalysis |
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