墨西哥玉米草组培体系的建立及愈伤组织切片观察

墨西哥玉米草是与玉米最近野生近缘种之一，可多茬刈割，产量高，品质好，被誉为青饲料之王，在畜牧业中扮演着重要角色，因此对墨西哥玉米草的深入研究具有重大的科研价值和经济效益．虽然墨西哥玉米草杂交体系已有较多报道，但墨西哥玉米草组培体系却鲜有研究．组培快速繁殖体系是通过生物技术对墨西哥玉米草进行品质改良的基础，因此建立高效的墨西哥玉米草组培快速繁殖体系具有重要意义．文中以墨西哥玉米草 8493 种子为实验材料，在含有2，4-D和6-BA的MS培养基上可以诱导幼苗基部的膨大节间，其在黑暗条件下两周可以诱导出愈伤组织．石蜡切片观察显示愈伤组织具备胚性愈伤特性．

In vitro proliferation and section observation for Teosinte

Though many studies about the hybridization between teosinte and closely related species of maize have been reported, little research about tissue culture of teosinte is reported. Here, establishment of technique system of tissue culture for teosinte was made by using the?variety 8493 of? Zea?mays?spp.mexicana?L as explants. The high totipotency swelling of the cultured internode was induced from teosinte seeds in the MS culture medium added 2,4-D and 6-BA and subcultured in the dark for induction of callus of teosinte about 2 weeks. The callus are authenticated to embryonic callus by paraffin section observation.