磁固相萃取与纳米金比色法联合检测蛋清中的溶菌酶

溶菌酶（LZ）在医药和工业方面有非常重要的应用，因此检测LZ的技术受到越来越多的关注. 我们建立了基于柠檬酸根包覆的纳米金（AuNPs）比色法对LZ进行快速测定. 当有LZ存在时，由于LZ与AuNPs的相互作用导致分散性良好的AuNPs发生聚集，从而使AuNPs溶液的颜色由红色变为蓝色. AuNPs溶液在625 nm和525 nm的吸光度比率（A625/A525）与LZ浓度在10-100 nM范围内有很好的线性相关关系，因此LZ的含量可以通过裸眼观测AuNPs溶液的颜色变化或用紫外可见分光光度计进行快速测定，LZ的最低检测限为10 nM. 我们将所建立的AuNPs比色法与磁固相萃取技术相结合对蛋清中LZ的含量进行了测定.

Colorimetric Detection of Lysozyme in Egg White using Gold Nanoparticles in Combination with Magnetic Solid Phase Extraction

Due to the industrial and medical importance of lysozyme (LZ), the LZ detection techniques have received more and more attention. Herein, we developed a simple colorimetric method for rapid LZ detection using citrate-capped gold nanoparticles without any further surface modification. The well-dispersed gold nanoparticles in aqueous solution were induced to aggregate in the presence of LZ, which led to a visible color change from red to blue. The concentration of LZ can be determined by monitoring with bare eye or a UV-vis spectrometer. The linearity was observed between the absorbance ratio (A625/A525) and the LZ concentration in the range of 10 to 100 nM. The lowest detectable concentration of LZ was 10 nM. The feasibility of the developed method was evaluated by determination of LZ in egg white in combination with magnetic solid phase extraction.