The role of kinesin, dynein and microtubules in pancreatic secretion |
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Authors: | Jürgen Schnekenburger Ina-Alexandra Weber Daniela Hahn Igor Buchwalow Burkhard Krüger Elke Albrecht Wolfram Domschke Markus M Lerch |
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Institution: | 1. Department of Medicine B, Westf?lische Wilhelms-University, Domagkstr. 3A, 48149, Münster, Germany 2. Gerhard Domagk Institute of Pathology, Westf?lische Wilhelms-University, Münster, 48149, Münster, Germany 3. Department of Pathology, University of Rostock, 18055, Rostock, Germany 4. Department of Muscle Biology and Growth, Research Institute for Biology of Farm Animals, 18196, Dummerstorf, Germany 5. Department of Medicine A, Ernst-Moritz-Arndt-University, Greifswald, 17487, Greifswald, Germany
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Abstract: | The regulated secretion of pancreatic zymogens depends on a functional cytoskeleton and intracellular vesicle transport. To
study the dynamics of tubulin and its motor proteins dynein and kinesin during secretion in pancreatic acinar cells, we infused
rats with 0.1 μg/kg/h caerulein. Electron and fluorescence microscopy detected neither dynein nor kinesin at the apical secretory
pole, nor on the surface of mature zymogen granules. After 30 min of secretagogue stimulation, kinesin and the Golgi marker
protein 58 K were reallocated towards the apical plasma membrane and association of kinesin with tubulin was enhanced. Disruption
of acinar cell microtubules had no effect on initial caerulein-induced amylase release but completely blocked secretion during
a second stimulus. Our results suggest that mature zymogen granule exocytosis is independent of intact microtubules, kinesin
and dynein. However, microtubule-dependent mechanisms seem to be important for the replenishment of secretory vesicles by
redistribution of Golgi elements towards the apical cell pole.
J. Schnekenburger and I.-A. Weber have contributed equally to this work. |
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Keywords: | Microtubules Motor proteins Pancreatic acinar cells Zymogen granule maturation Zymogen granule secretion |
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