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Isolation of a pollen specific promoter in tritordeum |
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| Authors: | Tu Zhiming Zhang Jiangzhou Chen Lin Chuan Qin Yang Guangxiao He Guangyuan |
| Institution: | Tu Zhiming1,Zhang Jiangzhou2,Chen Lin1,Chuan Qin1,Yang Guangxiao1,He Guangyuan1 (1. Key Laboratory of Molecular Biophysics of the Ministry of Education,China-UK Joint Lab,School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China,2. Department of Biochemistry and Molecular Biology,Medical College,Wuhan University,Wuhan 430071,China) |
| Abstract: | The promoter is a cis-acting element in regulating gene expression. A promoterless plasmid containing UidA gene was transformed into tritordeum by barmbadment. Histochemical analysis of various tissues in transgenic tritordeum was carried to examine tissue-specific expression of GUS(beta-glucuronidase) activity. The pollen-specific promoter was trapped and identified successfully in a transformant line. PCR(polymerase chain reaction) method was used to isolate this pollen-specific promoter. By sequencing an... |
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