烯丙胺等离子体处理聚丙烯膜的酶固定化

以烯丙胺等离子体对聚丙烯膜表面进行处理后,利用表面生成的氨基功能基团进行过氧化物酶的固定化,所采用的固定化方法主要有吸附法、戊二醛交联法和分子识别法.结果表明通过等离子体处理后聚丙烯膜表面的氨基基团可以有效地提高酶固定化效率,其中分子识别法可以得到具有最高酶活和酶稳定性的固定化酶膜.

Enzyme Immobilization on Allylamine Plasma-treated Polypropylene Membranes

Allylamine plasma was applied to the hydrophobic polypropylene microfiltration membranes to generate amino groups on membrane surface.These amino groups were later utilized to immobilize peroxidase onto membranes via various methods including physical adsorption,glutaraldehyde crosslinking and molecular recognition based on the interaction between biotin and avidin.The result reveals that compared with native polypropylene membrane,the surface modification with allylamine plasma can effectively improve the amount and storage stability as well as the working stability of immobilized peroxidase,among the various methods the molecular recognition method is proved to be the most efficient one.