| 重组人睫状神经营养因子突变体的制备及PEG修饰 |
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| 引用本文： | 韩莉,蔡志华,陶红梅,王进. 重组人睫状神经营养因子突变体的制备及PEG修饰[J]. 重庆师范大学学报(自然科学版), 2009, 26(2): 111-114 |
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| 作者姓名： | 韩莉 蔡志华 陶红梅 王进 |
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| 作者单位： | 重庆师范大学,生命科学学院,重庆,400047;重庆寰瑞生物技术有限公司,重庆,400030 |
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| 基金项目： | 重庆市科委自然科学基金 |
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| 摘 要： | 通过大肠杆菌重组表达人睫状神经营养因子突变体(CNTFm),并进行PEG修饰,旨在降低免疫原性.该突变体将天然CNTF的C端15个氨基酸删除,大肠杆菌表达的CNTFm以包含体形式存在,经复性、纯化获得纯度达到95%的目的蛋白.体内生物学活性测定结果显示,给药10 d,小鼠最大体重减少率达31%,产生的最高中和抗体滴度达到1:6 400; 经PEG修饰,CNTF突变体的生物学活性降低了34%,但最高中和抗体滴度降低到1:800.该PEG修饰后的CNTFm制备工艺有望为CNTF的临床应用开辟道路.
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| 关 键 词： | 睫状神经营养因子 原核表达 突变体 PEG修饰 肥胖 |
Preparation and PEGylation of Recombinant Human Ciliary Neurotrophic Factor Mutant |
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| HAN Li,CAI Zhi-hua,TAO Hong-mei,WANG Jin. Preparation and PEGylation of Recombinant Human Ciliary Neurotrophic Factor Mutant[J]. Journal of Chongqing Normal University:Natural Science Edition, 2009, 26(2): 111-114 |
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| Authors: | HAN Li CAI Zhi-hua TAO Hong-mei WANG Jin |
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| Affiliation: | 1.College of Life Sciences;Chongqing Normal University;Chongqing 400047;2.Chongqing Huanrui Biotechnologies Co. Ltd;Chongqing 400030;China |
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| Abstract: | Human ciliary neurotrophic factor(CNTF) is a neurotrophic factor(NTF) not included in NTF family. Recently body weight loss effect of CNTF was discovered in many studies, but clinical application has been limited as immunogenicity. Prepared ciliary neurotrophic factor mutant (CNTFm) with prokaryotic expression and analyzed effect on weight reduction. Chemical synthesis DNA of CNTFm, in which polypeptide is truncated 15aa at C-terminus. The DNA is subcloned in temperature and induced vetor pBV220, then the r... |
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| Keywords: | CNTF prokaryotic expression mutant PEGylation obesity |
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