| 高血糖条件下神经降压素调节巨噬细胞炎症反应的实验研究 |
| |
| 引用本文： | 张慧宇,郭敏芳,刘华,张艳霞. 高血糖条件下神经降压素调节巨噬细胞炎症反应的实验研究[J]. 山西大同大学学报(自然科学版), 2013, 0(6): 42-45 |
| |
| 作者姓名： | 张慧宇 郭敏芳 刘华 张艳霞 |
| |
| 作者单位： | [1]山西大同大学医学院中医系,山西大同037009 [2]山西大同大学医学院组胚撕室,山西大同037009 |
| |
| 基金项目： | 山西大同大学2011年度青年科学研究基金项目[2011Q13] |
| |
| 摘 要： | 目的观察神经降压素在高血糖条件下对巨噬细胞的影响。方法取昆明小鼠脾巨噬细胞，高糖培养液培养15d，细胞分PBS对照组和神经降压索干预组。ELISA检测法检测TNF-、IL-1β及IL-6的释放；RT—PCR检测TNF-、IL-1β及IL-6mRNA的表达。结果高糖条件下神经降压素能降低巨噬细胞TNF-、IL-1β及IL-6的释放和mRNA的表达（P〈0．05）。结论在高糖条件下，神经降压素可以通过抑制炎性细胞因子的释放影响巨噬细胞的反应。
|
| 关 键 词： | 高血糖 神经降压素 巨噬细胞炎症反应 |
The Experimental Study of Neurotensin Modulating the Inflammatory Response of Macrophages under Hyperglycemic Conditions |
| |
| Affiliation: | ZHANG Hui-yu, GUO Min-fang, LIU Hua, ZIIANG Yan-xia(1. Department of Tradilional Chinese Medicine, Medical School of Shanxi Datong University, Datong Shanxi, 037009; Department of Histology and Embryology, Medical School of Shanxi Datong University, Datong Shanxi, 037009) |
| |
| Abstract: | Objective To observe the effect of Neurotensin （NT） in macrophages function under hyperglycemic conditions. Methods Macrophages were prepared from spleen of Kunming mice and incubated in 30mM （high glucose） D-glucose, for 15 days. The ceils were divided into the PBS control group and Neurotensin treatment group. The level of TNF-α, IL-1β and IL-6 was detected by ELISA; The mRNA expressions of TNF-α, IL-1β and IL-6 were detected using RT-PCR. Results： NT can decrease the release of TNF-α, IL-1β and IL-6 in macrophages under hyperglycemic conditions （P〈0.05）. Conclusion These studies demonstrate that NT through the regulation of inflammatory cytokine expression affects macrophage responses. |
| |
| Keywords: | hyperglycemic neurotensin inflammatory response of macrophages |
| 本文献已被 维普 等数据库收录！ |
|
