| 新硫脲试剂同步荧光法测定蛋白质应用研究 |
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| 引用本文： | 崔凤灵,孔晓朵,卢雁,樊静,张贵生,李建平.新硫脲试剂同步荧光法测定蛋白质应用研究[J].河南师范大学学报(自然科学版),2009,37(1). |
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| 作者姓名： | 崔凤灵 孔晓朵 卢雁 樊静 张贵生 李建平 |
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| 作者单位： | 1. 河南师范大学,化学与环境科学学院,河南,新乡,453007
2. 鹤壁职业技术学院,医学院,河南,鹤壁,458030 |
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| 基金项目： | 国家自然科学基金，河南省高校科技创新团队支持计划 |
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| 摘 要： | 在模拟生理条件下,基于N-(4-乙氧苯基)-N'-(4-安替吡啉基)硫脲(EPAT)与牛血清白蛋白(BSA)的相互作用,BSA的同步荧光强度在一定范围内随BSA的浓度的增加而增强.据此建立了以EPAT为荧光分子探针,用同步荧光分析法测定BSA的新方法.在最佳实验条件下,EPAT-BSA体系的同步荧光强度在0～429.0μg·mL-1的浓度范围内与蛋白质浓度呈现良好的线性关系,相对标准偏差(RSD)为0.70%～1.61%,加标回收率在95.1%～103.5%之间.
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| 关 键 词： | N-(4-乙氧苯基)-N'-(4-安替吡啉基)硫脲(EPAT) 牛血清白蛋白(BSA) 同步荧光光谱 测定 |
Application Study of Synchronous Fluorescence in Protein Determination Using the Reagent of New Thiourea |
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| CUI Feng-ling,KONG Xiao-duo,LU Yan,FAN Jing,ZHANG Gui-sheng,LI Jian-ping.Application Study of Synchronous Fluorescence in Protein Determination Using the Reagent of New Thiourea[J].Journal of Henan Normal University(Natural Science),2009,37(1). |
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| Authors: | CUI Feng-ling KONG Xiao-duo LU Yan FAN Jing ZHANG Gui-sheng LI Jian-ping |
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| Institution: | 1.College of Chemistry and Environmental Science;Henan Normal University;Xinxiang 453007;China;2.Hebi Vocational & Technical College;Hebi 458030;China |
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| Abstract: | Under simulative biological conditions,upon the interaction between N-(4-ethoxyphenyl)-N'-(4-antipyrinyl)thiourea(EPAT)and bovine serum albumin(BSA),the research has studied the characterization of synchronous fluorescence spectra of EPAT-BSA system with EPAT as a molecular fluorescence probe.On basis of these,a new method for determination the proteins of serum albumin is developed with EPAT as a molecular fluorescence probe.Under the optimum experimental conditions,the synchronous fluorescence intensities... |
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| Keywords: | N-(4-ethoxyphenyl)-N'-(4-antipyrinyl)thiourea(EPAT) Bovine serum albumin(BSA) Synchronous fluorescence spectra determination |
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