丙酮酸转运体AtMPC3介导植物干旱胁迫响应机理研究

在干旱胁迫下,植物通过自身复杂而有效的应对机制减少水分散失,其中关闭植物气孔降低蒸腾作用是一个重要环节。脱落酸(ABA)是植物响应干旱胁迫产生的可以诱导气孔关闭、减少水分散失的重要植物激素。丙酮酸是光合作用糖酵解的产物,需要借助线粒体丙酮酸转运体(MPCs)进入线粒体中进行后续物质和能量代谢。本研究发现拟南芥线粒体丙酮酸转运体AtMPC3参与介导植物干旱胁迫响应,在外源施加ABA条件下,AtMPC3基因缺失突变体的气孔较野生型开度更小,植物失水率更低,表现出更强的抗旱能力。结果说明AtMPC3在脱落酸促进植物气孔关闭及干旱应答过程中的重要作用,对于提高植物抗旱能力以及作物产量提升具有潜在应用价值及重要意义。

Mitochondrial pyruvate carrier3 mediates abscisic acid-regulated stomatal closure and the drought response

Terrestrial plants have developed diverse mechanisms to save water especially in response to drought, and abscisic acid(ABA)is an essential phytohormone which could be induced by drought and promote stomatal closing to prohibit water loss as transpiration. Pyruvate is the product of glycolysis generated in cytosol, and could be transported by mitochondrial pyruvate carriers(MPCs)into mitochondrion for consequent cellular metabolism. The previous studies have shown that MPCs were involved in plant drought response. In the current study, we found that the functional null mutant of AtMPC3 was hypersensitive to ABA-induced stomatal closing to reduce water loss, and thus to facilitate the plant to survive the water deficiency. Our findings revealed the essential roles of AtMPC3 in ABA-promoted stomatal closing and plant drought resistance. These findings provide theoretical guidance to develop water-use-efficiency and high-yield modern agriculture.