HPLC-DAD法测定西藏产区金银花花蕾及叶中化学成分含量

采用高效液相色谱 二极管阵列检测法（HPLC DAD），对8批西藏产区的“亚特一号”良种金银花花蕾和叶中的活性成分进行了分析。测定了其中的8种主要化合物绿原酸、木犀草苷、马钱酸、隐绿原酸、马钱苷、断氧化马钱苷、芦丁、异绿原酸C的质量分数，分别为花蕾：3.85%、0.15%、0.18%、0.15%、0.11%、0.11%、0.17%和0.11%；叶：3.58%、0.79%、0.40%、0.08%、0.07%、0.24%、0.18%和0.05%。该研究为快速探明西藏产区良种金银花中的化学成分及进一步开发利用提供了方法和数据支持。

Determination of chemical components in flower buds and leaves of Lonicera japonica in Tibet area by HPLC-DAD

The active components of flower buds and leaves of Lonicera japonica “AT1”in Tibet area were analyzed to provide data support for their quality evaluation and exploitation. High performance liquid chromatography diode array detector (HPLC-DAD) was used to analyze 8 batches of Lonicera japonica Thunb. Flower buds and leaves in Tibet. The 8 main compounds of chlorogenic acid, luteoloside, loganin acid, cryptochlorogenic acid, loganin determination, Secoxyloganin,rutin, and isochlorogenic acid C content were measured, respectively. The buds were 3.85%, 0.15%,0.18%, 0.15%, 0.11%, 0.11%, 0.17% and 0.11%, and leaves: 3.58%, 0.79%,0.40%, 0.08%, 0.07%, 0.24%, 0.18% and 0.05%. This study provides a method and data support for the rapid detection ofchemical constituents and further exploitation of the fine species of Lonicera japonica in Tibet.