不同湿重诱导对毕赤酵母发酵生产植酸酶的影响

为了提高毕赤酵母工程菌（Mut+）植酸酶的表达量，对毕赤酵母工程菌50 L发酵生产植酸酶的工艺条件进行了研究。结果表明，通过流加葡萄糖提高菌体湿重到300 g/L，随后停止流加，溶氧回升后流加甲醇开始诱导，最终发酵结束时，植酸酶酶活达到21 666 U/mL，比对照提高12.9%，该诱导湿重有利于发酵生产植酸酶。

Influence of the wet cell weight before induction on secretive expression of phytase in Pichia pastoris

In order to increase the expression of phytase in Pichia pastoris, the fermentation conditions of phytase producing recombinant Pichia pastoris in 50 L fermentation tank were optimized. The results show that the strain wet weight at 300 g/L by feeding glucose, before addition of methanol, got the phytase activity 21 666 U/mL, 12.9% higher than the control by the end of fermentation, which is most favorable for the production of phytase by fermentation.