Functional analysis of hydrogenases and their effects on cell growth and magnetosome synthesis in <Emphasis Type="Italic">Magnetospirillum gryphiswaldense</Emphasis>

This study addressed the effect of hydrogen metabolism on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense strain MSR-1. Two deletion mutants were generated: L206, with single deletion of the hupL gene encoding H2-uptake NiFe] hydrogenase; and B206, with double deletion of the hyaB gene encoding H2-producing NiFe] hydrogenase and the hupL gene. The wild-type and mutant strains were compared in terms of hydrogen uptake capability, hydrogen yield, growth rate, and iron uptake, and o...