| 单克隆抗体制备中几个关键问题 |
| |
| 引用本文： | 王文聪. 单克隆抗体制备中几个关键问题[J]. 西昌学院学报(自然科学版), 2002, 0(2) |
| |
| 作者姓名： | 王文聪 |
| |
| 作者单位： | 西昌市畜牧局 四川西昌 |
| |
| 摘 要： | 随着分子生物学技术的进步,单克隆抗体的生产技术逐渐完善,但在生产中要制备出高质量的单抗仍存在许多问题。本文就动物的免疫、杂交瘤细胞的建立、融合细胞的筛选、克隆化培养及细胞冷藏、单克隆抗体的大量生产及纯化、单克隆抗体制备过程中的有关污染问题进行论述,以期对生产有一定的指导。
|
| 关 键 词： | 单克隆抗体 制备 关键 |
Key Problems in Production of Mono-Cloned Antibody |
| |
| Wang Wen-chong,Livestock Bureau of Xichang City,Xichang,Sichuan. Key Problems in Production of Mono-Cloned Antibody[J]. Journal of Xichang College, 2002, 0(2) |
| |
| Authors: | Wang Wen-chong Livestock Bureau of Xichang City Xichang Sichuan |
| |
| Affiliation: | Wang Wen-chong,Livestock Bureau of Xichang City,Xichang,Sichuan,615000 |
| |
| Abstract: | With the advancement of molecular biological technology, production of mono-cloned antibody is being improved. However many difficulties still exist in producing mono-cloned antibody of high quality. This paper covers animals" immunity, hybrid tumor cells, selection of merged cells, culture of cloning, refrigeration of cells, mass production and purification of mono-cloned antibody, and pollution during the production. |
| |
| Keywords: | Mono-Cloned Antibody Production Key |
| 本文献已被 CNKI 等数据库收录！ |
|
