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产脂肪酸菌种的筛选及部分酶学性质
引用本文:王艳茹,高贵,王师玉,王志,冯雁.产脂肪酸菌种的筛选及部分酶学性质[J].吉林大学学报(理学版),1999(4):2.
作者姓名:王艳茹  高贵  王师玉  王志  冯雁
作者单位:吉林大学酶工程实验室!长春,130023,吉林大学酶工程实验室!长春,130023,吉林大学酶工程实验室!长春,130023,吉林大学酶工程实验室!长春,130023,吉林大学酶工程实验室!长春,130023
摘    要:从含油污泥中筛选出一株产脂肪酶活力较高的微球菌,此菌最适产酶条件:2%的蛋白胨为氮源,1%的橄榄油为碳源,pH8.0,28℃培养48h.酶学性质研究发现,在28℃和45℃脂肪酶有较高催化活力,其中28℃活力更高些,最佳反应pH为8.0,pH稳定范围在6.0~10.0之间,Ca2 和K 对酶有激活作用,Fe2 ,Cu2 和Mn2 等对酶有抑制作用.

关 键 词:微球菌  脂肪酶  培养条件
修稿时间:: 1999-03

Selection of the Lipase-producing Strains and Some Properties of Partial Purified Lipase
Wang Yanru, Gao Gui, Wang Shlyu, Wang Zhi, Feng Yan.Selection of the Lipase-producing Strains and Some Properties of Partial Purified Lipase[J].Journal of Jilin University: Sci Ed,1999(4):2.
Authors:Wang Yanru  Gao Gui  Wang Shlyu  Wang Zhi  Feng Yan
Abstract:A mlcrococcus that produces lipase was isolated from soil by means of solid plate assay method. The optimal lipase-producing conditions of the strain were as follows: 1 % olive oil as carbon source, 2% peptone as nitrogen source, pH 8. 0, 28 C and the culture period of 48 h. The optimal pH and temperature for the hydrolysis of olive oil by partial purified lipase were pH 8. O and 28 C respectively. The enzyme;activity was stimulated by Ca2 and K but was inhibited by Ye2 , Cu2 and Mn2 etc.
Keywords:micrococcus  lipase  culture condition
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