| 假单胞菌质粒抗汞基因的克隆 |
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| 引用本文： | 周桢林,陈琦.假单胞菌质粒抗汞基因的克隆[J].中山大学学报(自然科学版),1990,29(3):129-135. |
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| 作者姓名： | 周桢林 陈琦 |
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| 作者单位： | 中山大学生物学系，中山大学生物学系 |
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| 摘 要： |
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| 关 键 词： | 抗汞基因 假单胞菌 基因 克隆 |
Cloning of the Hg-resistance Gene from the Plasmid pBH33 of Pseudomonas sp. |
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| Zhou Zhenlin,Chen Qi.Cloning of the Hg-resistance Gene from the Plasmid pBH33 of Pseudomonas sp.[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1990,29(3):129-135. |
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| Authors: | Zhou Zhenlin Chen Qi |
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| Institution: | Zhou Zhenlin;Chen Qi Department of Biology |
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| Abstract: | The Hg-resistance gene from the plasmid pBH33 of Pseudomonas sp.B-33 has been cloned in E.coli C_(600) using PstI digested plasmid pBR322 as vector.The molecular weight of the recombinant plasmid pBH337 was estimated to be about 6.1 kb according to EM and gel electrophoresis.The cloned gene located on a 1.8 kb PstI fragment of the plasmid pBH33 by restriction fragment analysis of the recombinant plasmid pBH337.Re-transformation suggested that the Hg-resistance gene was located on pBH337.Determination of growth showed that the recombin- ant C_(600)(pBH337)with Hg-resistance gene could grow very well in L-broth conta- ining 10ug/ml HgCl_2 and control C_(600) had strongly been repressed.The mercuric volatile of C_(600)(pBH337)was also markedly higher than that of the control C_(600) |
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| Keywords: | Hg-resistant gene Pseudomonas gene cloning |
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