| 影响水稻愈伤组织植株再生频率的因素研究 |
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| 引用本文： | 王力,张云孙,陈屹,高照,张虹,杜娟. 影响水稻愈伤组织植株再生频率的因素研究[J]. 云南大学学报(自然科学版), 1999, 21(2): 113-115,119 |
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| 作者姓名： | 王力 张云孙 陈屹 高照 张虹 杜娟 |
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| 作者单位： | 云南大学生物系,云南,昆明,650091 |
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| 摘 要： | 以水稻成熟种子为外植体，研究了影响水稻伤组织再生频率的各种因素，如渗透压，植物激素素和ＡｇＮＯ３等。发现不同基因型愈伤组织再生频率有很大差异，增加诱导培养基或分化培养基其中的渗透压和琼脂浓度，继代培养时加入适量的激动素或６－苄基氨基腺嘌呤以及在分化增减基中加入活性炭、玉米素等均可明显提高水稻愈伤组织的再生频率。
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| 关 键 词： | 愈伤组织 再生植株 水稻 再生频率 |
| 文章编号： | 0258-7971(1999)02-0113-03 |
Study on the Influence Factors of the Freguency of Plant Regeneration from Callus in Rice (Qryza sativa L.) |
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| WANG Li,ZHANG Yun-sun,CHEN Yi,GAO Zhao,ZHANG Hong,DU Juan. Study on the Influence Factors of the Freguency of Plant Regeneration from Callus in Rice (Qryza sativa L.)[J]. Journal of Yunnan University(Natural Sciences), 1999, 21(2): 113-115,119 |
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| Authors: | WANG Li ZHANG Yun-sun CHEN Yi GAO Zhao ZHANG Hong DU Juan |
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| Abstract: | The mature seeds of rice ( Qryza sativa L.) were used to study the influence factors of plant regeneration frequency of rice.The influence factors included osmotic pressure,plant hormone and AgNO 3 and so forth.The results showed that the frequency of plant regeneration varied greatly among different rice genotypes.Strengthening osmotic pressure and argar concentration in differentiation medium,supplement with activated charcoal (AC),zeatin (ZT) and AgNO 3 in differentiation medium have significantly increased the frequency of plant regeneration from callus in rice. |
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| Keywords: | mature seed of rice callus plant regeneration |
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