| 经典连苯三酚SOD测活法（420nm法）的某些影响因素 |
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| 引用本文： | 刘建荣,静天玉.经典连苯三酚SOD测活法（420nm法）的某些影响因素[J].河北大学学报(自然科学版),1995(1). |
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| 作者姓名： | 刘建荣 静天玉 |
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| 作者单位： | The Research Centre of Biotechnology，Hebei University，Baoding 071002 |
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| 摘 要： | 本文对超氧化物歧化酶（ＳＯＤ）的经典连苯三酚测活法的某些影响因素进行了考查。结果发现：固定自氧化速率，ｐＨ值改变０．１０单位将引起２０％左右的测定误差；固定连苯三酚终浓度，同样程度的ｐＨ变化仅产生约１３％的测定误差。在低浓度下（最终浓度小于０．１５ｍｍｏ１／Ｌ），连苯三酚的微小变化会严重影响活性测定结果。
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| 关 键 词： | 超氧化物歧化酶（SOD），超氧阴离子自由基（O＿2），连苯三酚自氧化法 |
Some Factors Influencing SoD Activity Assay in the Classical Pyrogallol Metbod (420nm Method) |
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| Liu Jianrong Jing Tianyu.Some Factors Influencing SoD Activity Assay in the Classical Pyrogallol Metbod (420nm Method)[J].Journal of Hebei University (Natural Science Edition),1995(1). |
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| Authors: | Liu Jianrong Jing Tianyu |
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| Abstract: | This paper examined some factors influencing superoxide dismutase(SOD) activity assay in the classicai pyrogallol method(420nm method).The resultsshowed that when the autoxidation rate was kept constant in the assay system,the pHchange of 0.10 unit could result in 20% error of the enzyme activity measured.If the finalconccntration of pyrogallol was kept constant,the pH change of same unit resulted in13%error of the activity. At low concentration of pyrogallol,say less than0.15mmol / L,the very little change of pyrogallol concentrations could seriously affectthe results of the assay. |
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| Keywords: | Superoxide dismutase(SOD)Superoxide free radical anion(O)Pyrogallol autoxidation method |
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