慢性阻塞性肺疾病西北寒燥证模型全身炎症反应状态研究

 通过测定慢性阻塞性肺疾病（Chronic Obstructive Pulmonary Disease，COPD）西北寒燥证模型外周血中炎症因子IL-1β、IL-8、IL-10、TNF-α含量的变化，揭示COPD西北寒燥证模型全身炎症反应状态。利用气管滴注弹性蛋白酶结合熏烟的方法建立COPD模型，在此基础上施以寒燥环境应激以建立COPD西北寒燥证模型。利用酶联免疫吸附测定（Enzyme-Linked Immunoadsordent Assay，ELISA）法测其外周血血清中IL-1β、IL-8、IL-10、TNF-α含量。结果表明，大鼠血清中弹性蛋白酶加熏烟加寒燥组（酶加CS加寒燥组）的IL-1β含量高于空白对照组（P＜0.05）；模型组IL-8、IL-10、TNF-α含量较空白对照组虽有增高趋势，但3组均不具有统计学差异。故而认为，西北寒燥证（寒燥）引起的全身炎症反应主要以增加血清中IL-1β含量为主，提示西北寒燥证具有加重COPD全身炎症的趋势，方域特色COPD的治疗应引起重视；西北寒燥证参与了COPD的发病，但其不是COPD中较重的证型，应尽早对其加以调控和预防。

Systemic Inflammatory Response in Cold Dry Syndrome of Chronic Obstructive Pulmonary Disease Model

By measuring the contents of inflammatory cytokines IL-1β, IL-8, IL-10 and TNF-α in peripheral area of cold dry syndrome of Chronic Obstructive Pulmonary Disease (COPD) model, the cold dry syndrome of the COPD model of systemic inflammatory response is characterized. The COPD model is established by combined cigarette smoke and tracheal instillation of elastase, and in pulse cold and dry environment, the cold dry syndrome of COPD is established. The Enzyme-Linked Immunosorbent Assay (ELISA) method is used to measure the blood serum of IL-1β, IL-8, IL-10 and TNF-α. The results show that in the serum, the cold and dry environment plus CS plus racheal instillation of elastase group makes the IL-1β content higher than that in the control group (P＜0.05); IL-8, IL-10 and TNF-α are in higher levels than in the control group, in general, but not with a significant difference between the three groups. So it may be concluded that the cold dry syndrome (cold and dry environment) leads to the systemic inflammatory response primarily due to the increase of IL-1β; the cold dry syndrome may increase the systemic inflammation level in COPD in some extent; in different areas, different treatments should be used; the cold dry syndrome takes part in COPD, but it does not belong to the heavy type of COPD, and, of course, it should be controlled and prevented.