| 利用RNA干扰沉默马尔尼菲青霉菌聚酮合酶基因的相关研究 |
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| 引用本文： | 赵允谦,陶菊红,陈小恂,郑爱霓,任双喜.利用RNA干扰沉默马尔尼菲青霉菌聚酮合酶基因的相关研究[J].复旦学报(自然科学版),2009,48(1). |
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| 作者姓名： | 赵允谦 陶菊红 陈小恂 郑爱霓 任双喜 |
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| 作者单位： | 赵允谦,ZHAO Yun-qian(复旦大学生命科学学院微生物学与微生物工程系,上海,200433);陶菊红,郑爱霓,任双喜,TAO Ju-hong,ZHENG Ai-ni,REN Shuang-xi(上海人类基因组研究中心,上海,201203);陈小恂,CHEN Xiao-xun(同济大学生命科学学院,上海,200092)
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| 摘 要： | 在实验室前期建立的马尔尼菲青霉菌cDNA文库中筛到了1个马尔尼菲青霉菌聚酮合酶基因cps(Cit-rinin Polyketide Synthase),为了研究这个cps基因的功能,构建了RNAi表达盒,表达盒内具有目的基因编码序列422 bp的反向重复序列并被gus基因隔开.整个干扰载体借助根癌农杆菌体系成功转化马尔尼菲青霉菌,并受木糖的诱导启动子xy/p调控RNA干扰.研究发现cps基因沉默的转化子产生与母代红色菌株不同的白色菌株,类似基因敲除的表型,表明cps基因与该青霉菌色素的形成直接相关.
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| 关 键 词： | RNA干扰 马尔尼菲青霉 聚酮合酶 色素 |
RNA Interference in Penicillium marneffei Demonstrates a Role for cps Gene in Pigmentation Biosynthesis |
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| ZHAO Yun-qian,TAO Ju-hong,CHEN Xiao-xun,ZHENG Ai-ni,REN Shuang-xi.RNA Interference in Penicillium marneffei Demonstrates a Role for cps Gene in Pigmentation Biosynthesis[J].Journal of Fudan University(Natural Science),2009,48(1). |
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| Authors: | ZHAO Yun-qian TAO Ju-hong CHEN Xiao-xun ZHENG Ai-ni REN Shuang-xi |
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| Institution: | 1.Department of Microbiology and Microbial Engineering;School of Life Sciences;Fudan University;Shanghai 200433;China;2.Chinese National Human Genome Center at Shanghai;Shanghai 201203;3.School of Life Sciences;Tongji University;Shanghai 200092;China |
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| Abstract: | To explore the gene function of cps gene,which encodes polyketide synthase and screened from cDNA library of Penicillium marneffei,a RNAi expression cassette was designed with inverted repeats of coding sequence of the targeted gene separated by a segment of gus gene.Constructs were transferred by Agrobacterium tumefaciesns-mediated transformation and under the control of promoter xylP,which promoter can be induced by xylose.The cps-silenced transformants demonstrated a clear white phenotype,instead of red ... |
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| Keywords: | RNAi Penicillium marneffei polyketide synthase pigmentation |
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