| 大孔树脂吸附和纯化蒲黄总黄酮的工艺研究 |
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| 引用本文： | 傅大煦,程小卫,王玮,陈家宽,周铜水.大孔树脂吸附和纯化蒲黄总黄酮的工艺研究[J].华东理工大学学报(自然科学版),2004,30(4):462-464. |
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| 作者姓名： | 傅大煦 程小卫 王玮 陈家宽 周铜水 |
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| 作者单位： | 1. 上海新药研究开发中心,上海,201203
2. 复旦大学生命科学学院,上海,200433 |
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| 摘 要： | 以主成分香蒲新苷及异鼠李素-3-O-新橙皮糖苷含量为指标，研究了HZ802非极性大孔树脂吸附和纯化蒲黄总黄酮的工艺条件。结果表明HZ802非极性大孔树脂对蒲黄总酮有良好的吸附纯化性能，其优化的工艺条件为：样品溶液的浓度为50mg／mL(生药量／体积)；树脂用量为生药量的1．5倍(质量比)；纯化先用2倍树脂柱体积的水，再用2倍树脂柱体积φ=0．2的乙醇；总黄酮的洗脱用2倍树脂柱体积φ=0．5的乙醇；吸附和洗脱流速均为2mL／min。
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| 关 键 词： | 蒲黄总黄酮 HZ802非极性大孔树脂 香蒲新苷 异鼠李素-3-O-新橙皮糖苷 |
| 文章编号： | 1006-3080(2004)04-0462-03 |
| 修稿时间： | 2004年3月24日 |
Absorption and Purification of the Total Flavonoids of Pollen typhae with Macro Resin |
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| FU Da-xu,CHENG Xiao-wei,WANG Wei,CHEN Jia-kuan,ZHOU Tong-shui.Absorption and Purification of the Total Flavonoids of Pollen typhae with Macro Resin[J].Journal of East China University of Science and Technology,2004,30(4):462-464. |
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| Authors: | FU Da-xu CHENG Xiao-wei WANG Wei CHEN Jia-kuan ZHOU Tong-shui |
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| Institution: | FU Da-xu~1,CHENG Xiao-wei~2,WANG Wei~2,CHEN Jia-kuan~2,ZHOU Tong-shui~ |
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| Abstract: | An optimum technique for absorbing and purifying the total flavonoids of Pollen typhae with HZ802 non-polarity macro resin was developed, using the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside as the evaluating criteria. The results showed that HZ802 non-polarity macro resin was suitable for absorbing and purifying the total flavonoid of Pollen typhae. The optimum technical operations were as follows:concentration of the sample was 50 mg/mL(weight of the raw material/volume); volume of the resin was 3 times weight of the raw material; purified with distilled water (2 times the volume of the resin), and then, with 20% ethanol (2 times the volume of the resin); 50% ethanol (2 times the volume of the resin) was used to elute the total flavonoids; both the current velocity of absoring and eluting were 2 mL/min. |
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| Keywords: | total flavonoids of Pollen typhae HZ802 non-polarity macro resin typhaneoside isorhamnetin-3-O-neohesperidoside |
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