| 改进CTAB法快速大量提取小菜蛾幼虫基因组DNA |
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| 引用本文： | 黄剑,吴文君. 改进CTAB法快速大量提取小菜蛾幼虫基因组DNA[J]. 贵州大学学报(自然科学版), 2005, 22(2): 181-183 |
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| 作者姓名： | 黄剑 吴文君 |
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| 作者单位： | 贵州大学,精细化工研究开发中心,贵州,贵阳,550025;西北农林科技大学,农药研究所,陕西,杨凌,712100 |
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| 摘 要： | 报道了一种为大量快速提取小菜蛾幼虫基因组DNA而改进的CTAB(溴代十六烷基三甲胺)法。研究结果表明，该方法比较简单、经济、有效，提取的基因组DNA完全能够满足PCR等分子生物学的研究分析。
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| 关 键 词： | CTAB法 提取 小菜蛾 基因组DNA |
| 文章编号： | 1000-5269(2005)02-0181-03 |
| 修稿时间： | 2005-03-02 |
A Rapid Improved CTAB Method for Macro-extraction Genomic DNA in the Larvae of Diamondback Moth, Plutella xylostella L. |
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| HUANG Jian,WU Wen-jun. A Rapid Improved CTAB Method for Macro-extraction Genomic DNA in the Larvae of Diamondback Moth, Plutella xylostella L.[J]. Journal of Guizhou University(Natural Science), 2005, 22(2): 181-183 |
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| Authors: | HUANG Jian WU Wen-jun |
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| Affiliation: | HUANG Jian 1,2,WU Wen-jun 2 |
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| Abstract: | A rapid improved CTAB (cetyltrimethyl ammonium bromide) for macro-extraction genomic DNA in the larvae of Plutella xylostella was reported. The method is a simple and efficient protocol for isolation of high molecular genomic DNA from insect. It showed that the resulting DNA is suitable for PCR and other molecular biology analysis. |
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| Keywords: | CTAB method extraction Plutella xylostella L. genomic DNA |
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