痕量检测中光源对荧光检测系统稳定性的影响

在荧光痕量检测中,光源的稳定性是影响荧光测量的重要因素,关系着整个检测系统的稳定及精度。针对石油污染物荧光检测设备,给出光源稳定性监测电路模型,并采用超前校正方法来消除转换过程由于相位而引起的自激振荡,实现光源的监测。通过实验研究了光源的不稳定性对系统性能的影响,从实验结果中分析出光源监测数据和荧光的检测数据呈现相关性,即光源的变化趋势与荧光的变化趋势一致,提出了统一量级归一处理方法并且应用到在线石油污染物荧光检测仪器中,提高了系统的稳定性。本研究可为荧光痕量检测中光源对检测结果影响的处理提供参考。

Effect of the Light Source on the Stability of the Fluorescence Detection System in Trace Detection

In the fluorescence trace detection system,the stability of the light source is one of an important factor,it relates to the stability and accuracy of the detection system.For the petroleum contaminants fluorescence detection equipment,the paper gives the monitoring circuit model of the light stability,and it realizes light monitoring by taking ahead of the correction method to eliminate the self-excited oscillation caused by the phase in the conversion process.It studied instability of the light which impacts on system performance by experiments.The light monitoring data and fluorescence detection data shows the correlation from the experimental results,the trend of light and fluorescence changes is identical.Putting forward the unified middleweight normalized approach and applied to the line of petroleum contaminants in fluorescence detection instrument,it improves the stability of the system.This study provides a reliable basis for the lighting effect in the fluorescence trace detection.