| 银杏茎段的组织培养 |
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| 引用本文： | 罗紫娟. 银杏茎段的组织培养[J]. 杭州师范学院学报(社会科学版), 1990, 0(3) |
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| 作者姓名： | 罗紫娟 |
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| 作者单位： | 杭州师范学院生物系 |
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| 摘 要： | 取自银杏(Ginkgo biloba Linn)雌雄株上当年抽出的幼嫩茎梢,剪去叶子,把嫩茎切成约0.5—0.8cm的具节小段,分别接种在十种培养基上。3天后切口处膨大;7—10天后,出现愈伤组织,节上的腋芽开始生长;30天左右培养于N_6+MS微量+kH_2PO_(4100)+2.4-D_3+BA_1+NAA_1PPm的培养基上的部分腋芽基部长出根,形成完整的再生植株。
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| 关 键 词： | 银杏 茎段培养 再生植株培养 |
TISSUE CULTRUE OF STEM SEGMENTS OF GINKGO BILOBA LINN |
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| Luo Zijuan. TISSUE CULTRUE OF STEM SEGMENTS OF GINKGO BILOBA LINN[J]. Journal of Hangzhou Teachers College(Humanities and Social Sciences), 1990, 0(3) |
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| Authors: | Luo Zijuan |
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| Affiliation: | Department of Biology |
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| Abstract: | The young stem tips of male and female Ginkge biloba Linn, were cut into 0.5-0.8cm stem segments and cultivated in ten different kinds of the culture medium. Callus were induced to form in 7-10 days. The axillary buds on the noded segments with nodes began to grow. Then in the culture medium: Ne+MStrace+KH2PO4100 + 2,4-D 3 +BA1+NAA1 PPm, roots were fromed from the bases of the axillary buds in about 30 days. These buds grew up to form complete plants. |
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| Keywords: | Ginkgo biloba Linn tissue cultrue of stem tissue cultrue regenerating plantlets. |
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