| 牛结核分枝杆菌PstS3基因克隆与序列分析 |
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| 引用本文： | 周晶,贾浩,徐广贤,王玉炯.牛结核分枝杆菌PstS3基因克隆与序列分析[J].宁夏大学学报(自然科学版),2010,31(3):273-274,279. |
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| 作者姓名： | 周晶 贾浩 徐广贤 王玉炯 |
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| 作者单位： | 宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川,750021 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目，高等学校科技创新工程重大项目 |
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| 摘 要： | 以牛结核分枝杆菌（Mycobacterium bovis）基因组DNA为模板,克隆PstS3基因,并进行序列测定,然后利用生物信息学软件对其序列进行分析.结果显示,PstS3基因全长1 113 bp,编码370个氨基酸,与GenBank所发表的人结核分枝杆菌PstS3基因的核苷酸同源性为99.82%,氨基酸同源性为99.4%,有1处位点发生有义突变.
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| 关 键 词： | 牛结核分枝杆菌 PstS3 序列分析 |
The PstS3 Gene Cloning and Sequence Analysis of Mycobacterium bovis |
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| Zhou Jing,Jia Hao,Xu Guangxian,Wang Yujiong.The PstS3 Gene Cloning and Sequence Analysis of Mycobacterium bovis[J].Journal of Ningxia University(Natural Science Edition),2010,31(3):273-274,279. |
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| Authors: | Zhou Jing Jia Hao Xu Guangxian Wang Yujiong |
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| Institution: | (Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan 750021,China) |
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| Abstract: | In order to study tuberculosis new subunits vaccine,a PCR strategy is performed by using M.bovis DNA as the template and then amplifying PstS3 genes,which is cloned into PMD18-T and sequenced,then the nucleotide sequence of positive recon is analyzed by DNAMAN.The results show that the total length of PstS3 is 1 113 bp,encoded 370 amino acids;compared with PstS3 sequence published on Genbank,the nucleotide homology is 99.82%,while the amino acid homology is 99.4%.There are one situs which take place plus sense mutation. |
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| Keywords: | PstS3 |
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