| 马铃薯转基因株系载体骨架的整合分析 |
| |
| 引用本文： | 成善汉,谢从华,柳俊,宋波涛,吴艳阁.马铃薯转基因株系载体骨架的整合分析[J].海南大学学报(自然科学版),2006,24(3):305-308,316. |
| |
| 作者姓名： | 成善汉 谢从华 柳俊 宋波涛 吴艳阁 |
| |
| 作者单位： | 海南大学,生命科学与农学院,海南,海口,570228;华中农业大学,园艺植物生物学教育部重点实验室,湖北,武汉,430070 |
| |
| 摘 要： | 对衍生于常用载体pBI 121的4种表达载体所获得的马铃薯转基因株系进行了PCR骨架整合分析,结果表明,左边界序列和短片段骨架基因更容易整合进基因组中,转入基因组的载体骨架片段的大小也不一样.这说明在转基因植株的检测中要注意骨架载体是否转入,以便消除生物安全性问题.
|
| 关 键 词： | 转基因植株 载体骨架 整合 PCR |
| 文章编号： | 1004-1729(2006)03-0305-04 |
| 修稿时间： | 2005年12月2日 |
Integration Analysis on Vector Backbone of Transgenic Potato Lines |
| |
| CHENG Shan-han,XIE Cong-hua,LIU Jun,SONG Bo-tao,WU Yan-ge.Integration Analysis on Vector Backbone of Transgenic Potato Lines[J].Natural Science Journal of Hainan University,2006,24(3):305-308,316. |
| |
| Authors: | CHENG Shan-han XIE Cong-hua LIU Jun SONG Bo-tao WU Yan-ge |
| |
| Abstract: | The PCR method is employed to analyze transgenic potato lines transformed by four different vectors that were derived from common vector pBI121.The results showed the sequences of LB and the shorter genes of vector backbone are integrated into potato genome easier and the size of vector backbone fragment integrated into genome of potato are different.The results demonstrate that it is necessary to detect transgenic lines in order to eliminate the problem about biological security. |
| |
| Keywords: | Transgenic lines vector backbone integration PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
